RT info:eu-repo/semantics/article
T1 The status of EGFR modulates the effect of miRNA-200c on ZEB1Expression and cell migration in glioblastoma cells.
A1 Muñoz-Hidalgo, Lisandra
A1 San-Miguel, Teresa
A1 Megías Vericat, Javier
A1 Serna García, Eva
A1 Calabuig-Fariñas, Silvia
A1 Monleón, Daniel
A1 Gil-Benso, Rosario
A1 Cerdá-Nicolás, Miguel J.
A1 López Ginés, Concha
K1 Glioblastoma
K1 EGFR amplification
K1 miR-200c
K1 ZEB1
K1 Cell migration
AB Migration of glioblastoma cells into surrounding tissue is one of the main features that makes this tumor incurable. We evaluated whole-genome miRNA expression profiling associated with different EGFR amplification patterns in 30 cases of primary glioblastoma. From the 64 miRNAs that showed differential expression between tumors with a high level of EGFR amplification and tumors without EGFR amplification, 40% were related with cell migration, being miR-200c the most differentially expressed between these two groups. We investigated the effect of miR-200c on ZEB1 expression and cell migration in an in vitro transfection model with a miR-200c mimic, a miR-200c inhibitor and siRNA targeting EGFR in three short-term cultures with different levels of EGFR amplification obtained from resected glioblastomas. The cell culture with the highest EGFR amplification level presented the lowest miR-200c expression and the status of EGFR modulated the effect of miR-200c on ZEB1 expression. Silencing EGFR led to miR-200c upregulation and ZEB1 downregulation in transfected cultures, except in the presence of high levels of EGFR. Likewise, miR-200c upregulation decreased ZEB1 expression and inhibited cell migration, especially when EGFR was not amplified. Our results suggest that modulating miR-200c may serve as a novel therapeutic approach for glioblastoma depending on EGFR status.
SN 1422-0067
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/36171
UL http://riull.ull.es/xmlui/handle/915/36171
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 10-jun-2024