Prevalence and Characterisation of Multiresistant Bacterial Strains Isolated in Pigs from the Island of Tenerife
Fecha
2022Resumen
Background: Antibiotic-resistant bacteria can circulate among human and animal populations through direct contact with animals, as well as via food and the environment. The purpose of
this study was to examine the prevalence and characterisation of multiresistant bacteria in pig samples.
Methods: 224 samples of pig livestock were taken at the slaughterhouse on the island of Tenerife. A
nasal and a rectal sample were collected from each pig. The presence of methicillin-resistant Staphylococcus aureus (MRSA), methicillin-resistant Staphylococcus coagulase-negative (MRCoNS), vancomycinresistant Enterococcus (VRE), extended-spectrum ß-lactamase-producing Enterobacteriaceae (BLEE),
carbapenemase-producing Enterobacteriaceae (CPE), and colistin-resistant Enterobacteriaceae was
investigated. The resistance genes of the isolated bacteria were characterised by specific PCRs depending on the microorganism to be studied, and in vitro antimicrobial resistance was determined using
the broth microdilution method (Vitek®2 system bioMérieux®, Nurtingen, Germany). Results: MRSA
prevalence was 73.21% (164 isolates). MRCoNS prevalence was 9.8% (22 isolates), S. sciuri being
the prevalent species. Six isolates presented a 2.7% prevalence of extended-spectrum ß-lactamaseproducing Escherichia coli (BLEE) in the CTX-M-1 group. No vancomycin-resistant Enterococcus
(VRE), carbapenemase-producing Enterobacteriaceae (CRE), or colistin-resistant Enterobacteriaceae were
isolated. Conclusion: we found a high presence of multiresistant bacteria, suggesting the need for
increased control and surveillance of this type of strains in pig livestock and a better understanding
of the possible transmission routes of these microorganisms through livestock products.