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dc.contributor.authorBarrios del Pino, Yvelise 
dc.contributor.authorRodriguez, Aurelio
dc.contributor.authorFranco, Andrés
dc.contributor.authorAlava-Cruz, Cristina
dc.contributor.authorMarrero-Miranda, Domingo
dc.contributor.authorPerez-Tamajon, Lourdes
dc.contributor.authorMatheu, Victor
dc.date.accessioned2023-12-19T21:06:07Z
dc.date.available2023-12-19T21:06:07Z
dc.date.issued2021
dc.identifier.issn2076-393X
dc.identifier.urihttp://riull.ull.es/xmlui/handle/915/34890
dc.description.abstractPreviously, the delayed-type hypersensitivity (DTH) cutaneous test with the spike protein of SARS-CoV-2 has been shown to be a simple in vivo method to measure T-cell functionality after natural infection and in vaccinated individuals. Methods: Twenty-five kidney-transplanted recipients were immunized with two doses of the mRNA-based Pfizer–BioNTech COVID19 vaccine three weeks apart. Cell-immune response (CIR) was evaluated ten weeks later using an in vivo DTH skin test and in vitro with an interferon gamma release assay (IGRA). Humoral Immune Response (HIR) was determined by the measurement of specific IgG anti-S1 SARS-CoV-2. Results: Ten weeks after the second dose of the vaccine, 23 out of 25 transplanted patients had a positive DTH skin test, while in vitro CIR was considered positive in 20 patients. Unspecific stimulation was positive in all 25 patients, showing no T-cell defect. Seven out of twenty-five patients had a negative specific anti-spike IgG. CIR was positive in all immune-competent control patients. Conclusions: DTH is a useful, simple, and cheaper tool that can be used to assess cellular immune response, with an excellent correlation with the in vitro CIR. CIR assessment after vaccination in these immunocompromised patients is an excellent complement to HIR-based methods. This skin test could be used if classical in vitro methods cannot be applieden
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.relation.ispartofseriesVaccines 2021, 9, 1315
dc.rightsLicencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES
dc.titleOptimizing a Protocol to Assess Immune Responses after SARS-CoV-2 Vaccination in Kidney-Transplanted Patients: In Vivo DTH Cutaneous Test as the Initial Screening Method
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.3390/vaccines9111315


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