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PI4P5-Kinase Ialpha Is Required for Efficient HIV-1 Entry and Infection of T Cells.
dc.contributor.author | Valenzuela Fernández, Agustín | |
dc.contributor.author | Barrero Villar, Marta | |
dc.contributor.author | Barroso Gonza´lez, Jonathan | |
dc.contributor.author | Cabrero, J. R. | |
dc.contributor.author | Gordón Alonso, Mónica | |
dc.contributor.author | Álvarez Losada, Susana | |
dc.contributor.author | Muñoz Fernández, M. A. | |
dc.contributor.author | Sánchez Madrid, Francisco | |
dc.contributor.other | Medicina Física y Farmacología | |
dc.contributor.other | Grupo "Inmunología Celular y Viral". | |
dc.date.accessioned | 2024-01-15T21:08:56Z | |
dc.date.available | 2024-01-15T21:08:56Z | |
dc.date.issued | 2008 | |
dc.identifier.uri | http://riull.ull.es/xmlui/handle/915/35356 | |
dc.description.abstract | HIV-1 envelope (Env) triggers membrane fusion between the virus and the target cell. The cellular mechanism underlying this process is not well known. Phosphatidylinositol 4,5-bisphosphate (PIP2) is known to be important for the late steps of the HIV-1 infection cycle by promoting Gag localization to the plasma membrane during viral assembly, but it has not been implicated in early stages of HIV-1 membrane-related events. In this study, we show that binding of the initial HIV-1 Env-gp120 protein induces PIP2 production in permissive lymphocytes through the activation of phosphatidylinositol-4-phosphate 5-kinase (PI4P5-K) I!. Overexpression of wild-type PI4P5-K I! increased HIV-1 Env-mediated PIP2 production and enhanced viral replication in primary lymphocytes and CEM T cells, whereas PIP2 production and HIV-1 infection were both severely reduced in cells overexpressing the kinase-dead mutant D227A (D/A)-PI4P5-K I!. Similar results were obtained with replicative and single-cycle HIV-1 particles. HIV-1 infection was also inhibited by knockdown of endogenous expression of PI4P5-K I!. These data indicate that PI4P5-K I!-mediated PIP2 production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | |
dc.relation.ispartofseries | The Journal of Immunology, 2008, 181: 6882– 6888 | |
dc.rights | Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional) | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES | |
dc.title | PI4P5-Kinase Ialpha Is Required for Efficient HIV-1 Entry and Infection of T Cells. | en |
dc.type | info:eu-repo/semantics/article |