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dc.contributor.authorValenzuela Fernández, Agustín 
dc.contributor.authorBarrero Villar, Marta
dc.contributor.authorCabrero, José Román
dc.contributor.authorGordón Alonso, Mónica
dc.contributor.authorBarroso González, Jonathan
dc.contributor.authorÁlvarez Losada, Susana
dc.contributor.authorMuñoz Fernández, M. Ángeles
dc.contributor.authorSánchez Madrid, Francisco
dc.contributor.otherMedicina Física y Farmacología
dc.contributor.otherGrupo "inmunología Celular y Viral".
dc.date.accessioned2024-01-15T21:10:01Z
dc.date.available2024-01-15T21:10:01Z
dc.date.issued2009
dc.identifier.urihttp://riull.ull.es/xmlui/handle/915/35368
dc.description.abstractThe human immunodeficiency virus 1 (HIV-1) envelope regulates the initial attachment of viral particles to target cells through its association with CD4 and either CXCR4 or CCR5. Although F-actin is required for CD4 and CXCR4 redistribution, little is known about the molecular mechanisms underlying this fundamental process in HIV infection. Using CD4+ CXCR4+ permissive human leukemic CEM T cells and primary lymphocytes, we have investigated whether HIV-1 Env might promote viral entry and infection by activating ERM (ezrin-radixin-moesin) proteins to regulate F-actin reorganization and CD4/CXCR4 co-clustering. The interaction of the X4-tropic protein HIV-1 gp120 with CD4 augments ezrin and moesin phosphorylation in human permissive T cells, thereby regulating ezrin-moesin activation. Moreover, the association and clustering of CD4-CXCR4 induced by HIV-1 gp120 requires moesin-mediated anchoring of actin in the plasma membrane. Suppression of moesin expression with dominant-negative N-moesin or specific moesin silencing impedes reorganization of F-actin and HIV-1 entry and infection mediated by the HIV-1 envelope protein complex. Therefore, we propose that activated moesin promotes F-actin redistribution and CD4-CXCR4 clustering and is also required for efficient X4-tropic HIV-1 infection in permissive lymphocytes.en
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.relation.ispartofseriesJournal of Cell Science 122, 103-113
dc.rightsLicencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES
dc.titleMoesin is required for HIV-1-induced CD4-CXCR4 interaction, F-actin redistribution, membrane fusion and viral infection in lymphocytes.en
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1242/jcs.035873
dc.subject.keywordERMen
dc.subject.keywordEzrin-radixin-moesinen
dc.subject.keywordF-actin redistributionen
dc.subject.keywordCD4-CXCR4 interactionen
dc.subject.keywordHIV-1 infection and entryen


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