Immunometabolism is a key factor for the persistent spontaneous elite control of HIV-1 infection.
Date
2019Abstract
Background: Approximately 25% of elite controllers (ECs) lose their virological control by
mechanisms that are only partially known. Recently, immunovirological and proteomic factors have
been associated to the loss of spontaneous control. Our aim was to perform a metabolomic approach
to identify the underlying mechanistic pathways and potential biomarkers associated with this loss
of control.
Methods: Plasma samples from EC who spontaneously lost virological control (Transient Controllers,
TC, n = 8), at two and one year before the loss of control, were compared with a control group of
EC who persistently main- tained virological control during the same follow-up period (Persistent
Controllers, PC, n = 8). The determination of metabolites and plasma lipids was performed by
GC-qTOF and LC-qTOF using targeted and untargeted ap- proaches. Metabolite levels were associated
with the polyfunctionality of HIV-specific CD8⁺T-cell response.
Findings: Our data suggest that, before the loss of control, TCs showed a specific circulating
metabolomic profile characterized by aerobic glycolytic metabolism, deregulated mitochondrial
function, oxidative stress and increased immunological activation. In addition, CD8⁺ T-cell
polyfunctionality was strongly associated with me- tabolite levels. Finally, valine was the main
differentiating factor between TCs and PCs.
Interpretation: All these metabolomic differences should be considered not only as potential
biomarkers but also
as therapeutic targets in HIV infection.