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The status of EGFR modulates the effect of miRNA-200c on ZEB1Expression and cell migration in glioblastoma cells.
dc.contributor.author | Muñoz-Hidalgo, Lisandra | |
dc.contributor.author | San-Miguel, Teresa | |
dc.contributor.author | Megías Vericat, Javier | |
dc.contributor.author | Serna García, Eva | |
dc.contributor.author | Calabuig-Fariñas, Silvia | |
dc.contributor.author | Monleón, Daniel | |
dc.contributor.author | Gil-Benso, Rosario | |
dc.contributor.author | Cerdá-Nicolás, Miguel J. | |
dc.contributor.author | López Ginés, Concha | |
dc.date.accessioned | 2024-02-08T21:07:12Z | |
dc.date.available | 2024-02-08T21:07:12Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://riull.ull.es/xmlui/handle/915/36171 | |
dc.description.abstract | Migration of glioblastoma cells into surrounding tissue is one of the main features that makes this tumor incurable. We evaluated whole-genome miRNA expression profiling associated with different EGFR amplification patterns in 30 cases of primary glioblastoma. From the 64 miRNAs that showed differential expression between tumors with a high level of EGFR amplification and tumors without EGFR amplification, 40% were related with cell migration, being miR-200c the most differentially expressed between these two groups. We investigated the effect of miR-200c on ZEB1 expression and cell migration in an in vitro transfection model with a miR-200c mimic, a miR-200c inhibitor and siRNA targeting EGFR in three short-term cultures with different levels of EGFR amplification obtained from resected glioblastomas. The cell culture with the highest EGFR amplification level presented the lowest miR-200c expression and the status of EGFR modulated the effect of miR-200c on ZEB1 expression. Silencing EGFR led to miR-200c upregulation and ZEB1 downregulation in transfected cultures, except in the presence of high levels of EGFR. Likewise, miR-200c upregulation decreased ZEB1 expression and inhibited cell migration, especially when EGFR was not amplified. Our results suggest that modulating miR-200c may serve as a novel therapeutic approach for glioblastoma depending on EGFR status. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | |
dc.relation.ispartofseries | International Journal Molecular Sciences, 2021, 22(1) | |
dc.rights | Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional) | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES | |
dc.title | The status of EGFR modulates the effect of miRNA-200c on ZEB1Expression and cell migration in glioblastoma cells. | |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | 10.3390/ijms22010368 | |
dc.subject.keyword | Glioblastoma | en |
dc.subject.keyword | EGFR amplification | en |
dc.subject.keyword | miR-200c | en |
dc.subject.keyword | ZEB1 | en |
dc.subject.keyword | Cell migration | en |
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DBIOQ. Bioquímica, Microbiología, Biología Celular y Genética
Documentos de investigación (artículos, libros, capítulos de libros, ponencias...) publicados por investigadores del Departamento de Bioquímica, Microbiología, Biología Celular y Genéica