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RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis.
| dc.contributor.author | Ortega Rivas, Antonio | |
| dc.contributor.author | Martínez Carretero, Enrique | |
| dc.contributor.author | Alonso, V. | |
| dc.contributor.author | Quispe, A. | |
| dc.contributor.author | Thomas, M.C . | |
| dc.contributor.author | Alonso, R. | |
| dc.contributor.author | Piñero Barroso, José Enrique | |
| dc.contributor.author | González, A.C. | |
| dc.contributor.author | Valladares Hernández, Basilio | |
| dc.contributor.other | Didácticas Específicas | |
| dc.contributor.other | Instituto de Enfermedades Tropicales y Salud Pública de Canarias | |
| dc.date.accessioned | 2024-09-23T20:05:13Z | |
| dc.date.available | 2024-09-23T20:05:13Z | |
| dc.date.issued | 2003 | |
| dc.identifier.uri | http://riull.ull.es/xmlui/handle/915/38839 | |
| dc.description.abstract | The technique of Random Amplification Polymorphic DNA allows fragments of the genome to be amplified by means of polymerase chain reaction (PCR) without previous knowledge of their sequences. The protozoa of the genus Leishmania present great genetic variability, making it difficult to characterize the different species. A method is developed with a single 10-mers long primer, which allows the species L. braziliensis, L. mexicana, L. infantum, L. tropica, L. chagasi, L. amazonensis and L. major to be differentiated. These products amplified by RAPD have also facilitated the design of some primers that amplify L. braziliensis DNA exclusively. | en |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | Parasitology (2003), 127 | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.title | RAPD method useful for distinguishing Leishmania species: design of specific primers for L. braziliensis. | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | 10.1017/S0031182003004104 | |
| dc.subject.keyword | RAPD | |
| dc.subject.keyword | PCR | |
| dc.subject.keyword | primer | |
| dc.subject.keyword | Leishmania braziliensis |