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Design and evaluation of a specific primer pair for the diagnosis and identification of Acanthamoeba polyphaga
| dc.contributor.author | Ortega Rivas, Antonio | |
| dc.contributor.author | Lorenzo Morales, Jacob | |
| dc.contributor.author | Martínez Carretero, Enrique | |
| dc.contributor.author | Villa Lloberas, Mercedes | |
| dc.contributor.author | Valladares Hernández, Basilio | |
| dc.contributor.author | del Castillo Remiro, Antonio | |
| dc.contributor.other | Didácticas Específicas | |
| dc.contributor.other | Instituto de Enfermedades Tropicales y Salud Pública de Canarias | |
| dc.date.accessioned | 2024-09-23T20:06:48Z | |
| dc.date.available | 2024-09-23T20:06:48Z | |
| dc.date.issued | 2004 | |
| dc.identifier.uri | http://riull.ull.es/xmlui/handle/915/38857 | |
| dc.description.abstract | Random amplified polymorphism DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that may be useful for species diagnosis. In this study, a distinctive a 962-bp band in A. polyphaga band patterns was found, by using the OPC20 primer (ACTTCGCCAC). The DNA fragment was used to design a specific primer pair that was useful for the identification of different isolates as A. polyphaga species. A case of A. polyphaga in disseminated acanthamoebiasis affecting mesenteric nodes is also reported. | en |
| dc.format.mimetype | application/pdf | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | Current Microbiology, Vol. 48 (2004) | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.title | Design and evaluation of a specific primer pair for the diagnosis and identification of Acanthamoeba polyphaga | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.identifier.doi | 10.1007/s00284-003-4211-5 |