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dc.contributor.authorOrtega Rivas, Antonio 
dc.contributor.authorLorenzo Morales, Jacob 
dc.contributor.authorAlonso, Violeta
dc.contributor.authorAbreu Acosta, Néstor 
dc.contributor.authorForonda, Pilar 
dc.contributor.authordel Castillo Remiro, Antonio 
dc.contributor.authorValladares Hernández, Basilio 
dc.contributor.otherDidácticas Específicas
dc.contributor.otherInstituto de Enfermedades Tropicales y Salud Pública de Canarias
dc.date.accessioned2024-09-23T20:07:35Z
dc.date.available2024-09-23T20:07:35Z
dc.date.issued2003
dc.identifier.urihttp://riull.ull.es/xmlui/handle/915/38866
dc.description.abstractIn the present study, we demonstrated the Random Amplified Polymorphism DNA (RAPD) diagnostic validity. In our study, we have analyzed RAPD profiles searching for characteristic and useful bands for Acanthamoeba diagnosis at the species level. We found a distinctive 370-bp band in A. divionensis RAPD patterns, using the OPC14 primer (TGCGTGCTTG) (Operon Technologies, Inc., Alameda, CA). The band specificity was confirmed by hybridization, using it as a probe, against all OPC14 amplifications from 10 different Acanthamoeba species. Once we sequenced this band, we used it to design a specific primer pair which showed positive amplification only in A. divionensis isolates.en
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.relation.ispartofseriesCurrent Microbiology, Vol. 47 (2003)
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleRandom amplified polymorphic DNA profiles as a tool for the identification of Acanthamoeba divionensisen
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doi10.1007/s00284-002-3939-7


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