RT info:eu-repo/semantics/masterThesis
T1 Screening de variantes genéticas de resistencia en Aedes aegypti de la Isla de Santiago (Cabo Verde).
A1 De Carvalho Correia, Wilson Silvestre
A2 Máster Univ. en Investigación y Diagnóstico de Enfermedades Tropicales
K1 Gen COI
K1 Ae. aegypti
K1 Piretroides
K1 Deltametrina
AB The Aedes aegypti mosquito is considered one of the most important vectors fortransmitting the Dengue, Zika and Chikungunya virus. In Cape Verde this species ispresent in all the Islands and is currently considered the vector of dengue and zika in thecountry. A part of the control of these diseases focuses on the use of insecticides. Theemergence of resistance mutations to pyrethroids in Ae. aegypti has been widelydocumented. Previous studies on Cape Verde Ae. aegypti have shown deltamethrinresistant phenotype but the profile of mutations resistant to this pyrethroid is unknown.The aim of this work is to carry out a preliminary study of the genetic sensitivity profileof the Ae. aegypti of the Santiago Island to this pyrethroid, first, evaluating the geneticvariability of mosquitoes and then, looking for mutations Kdr involved in resistance tothis insecticide. For this, DNA was extracted from adult mosquitoes (N = 12), capturedin the north, center and south of the Island, from which the 5' region of mitochondrialcytochrome c oxidase (COI) gene and two regions of sodium channel gene were amplifiedto search the mutations of interest. The analysis of the COI gene showed a lownucleotidique diversity and divergence among individuals, as well as all of them belongedto the haplogroup II. All this possibly pointing to a possible recent colonization and fromone place. Individuals from different areas of the island shared the same haplotype, whichalso indicates a scarce differentiation between samples from different locations.Regarding deltamethrin resistance, we did not detect any of the Kdr mutations genenormally related to this resistance (S989P, V1016G and F1534C). However, this does notrule out the presence of the other mutations in this or other genes that would explain theresistance found in this population of mosquitoes.
YR 2018
FD 2018
LK http://riull.ull.es/xmlui/handle/915/10902
UL http://riull.ull.es/xmlui/handle/915/10902
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 22-nov-2024