RT info:eu-repo/semantics/masterThesis
T1 Análisis transcripcional de la respuesta inmune en órganos diana de la leishmaniasis
A1 Palacios Cortés, Génesis
A2 Máster Univ. en Investigación y Diagnóstico de Enfermedades Tropicales
K1 Leishmaniasis visceral
AB The study of the immunological mechanisms in the infection by Leishmania sp, requires theevaluation of the gene expression in vivo, many studies have provided information about whathappens at the in vitro level, but the knowledge of the changes at the transcriptome level using themurine model, from tissue of the affected organ, in this case the liver, would allow to obtain anoverview of gene expression and a better approach to the immune response in humans. Therefore,in the present study it was proposed to know the gene expression in a first moment of the infection(at 24 hours), from liver tissue of BALB/c mice infected with L. infantum, and samples fromuninfected mice as control group. From the RNA extraction and with the retrotranscriptionproducts, High-throughput Real-time quantitative PCR was performed to know the expression of112 genes related to the immune response. Among the main findings, it is possible to highlight that24 hours post infection predominates the expression of genes involved in the inflammatoryresponse of macrophage activation (M1) where Stat1 seems to have a central role in the innateimmune response, since it connects the genes involved in the production of IFN-γ with the genesrelated to leukocyte chemotaxis. At this time of infection, chemotactic signals have already beengenerated for T lymphocytes, NK cells and monocytes. Additionally, the detection of costimulatorymolecules such as CD40/CD40LG would indicate that activation of CD4+ T lymphocytes is takingplace. It also highlights the overexpression of Ifng and Nos2, which It indicates that it would beproducing macrophage activation by the classical pathway, important for infection controlmechanism in the liver.
YR 2018
FD 2018
LK http://riull.ull.es/xmlui/handle/915/11611
UL http://riull.ull.es/xmlui/handle/915/11611
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 22-nov-2024