RT info:eu-repo/semantics/bachelorThesis
T1 Anisotropy and lifetime decay of green fluorescent protein in glycerol
A1 Brauner, Maren
AB Fluorescence is a phenomena that can be observed on the surface of tonic water in sunlight, perceiving the emission of blue light from a substance calledquinine. In 1845 Sir John F. W. Herschel [1] discovered the previous phenomena. Nowadays, fluorescence embodies a useful research tool in variousscientific fields such as biology, not only acting as a stain for diverse applications such as tracing cell organelles but also giving presumably importantinformation about its environment such as the viscosity of the medium. Thiswork is focused on the green fluorescent protein (GFP) with the objective tomeasure its emission spectra, its lifetime and anisotropy decay. According tothe executed experiment, the result for the emission peak is, located in thegreen wavelength range at λem = 510 nm. The lifetime decay also showsthe expected behavior, involving the effects that occur during the excitationperiod. For the anisotropy decay measurements, the objective is to comparethe measuring results with different models for the three dimensional shapeof the GFP. Knowing a term called rotational correlation time θ from the experiment, the volume V of the protein can be determined. If the volume isdefined and θ is measured, the viscosity of the medium that surrounds theGFP can be found. This part of the research was complicated as not all of theobtained data followed the expectations, hence, both models and ideas howto improve the procedure will be discussed theoretically in more detail.
YR 2019
FD 2019
LK http://riull.ull.es/xmlui/handle/915/15728
UL http://riull.ull.es/xmlui/handle/915/15728
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 27-nov-2024