RT info:eu-repo/semantics/bachelorThesis
T1 Evaluación de ensayos de citotoxicidad frente a Redes Metal-Orgánicas
A1 Cerro Muñoz, Sandra
A1 Pino Estévez, Verónica
K1 Redes metal-orgánicas, MOFs, Citotoxicidad, AlamarBlue®, Macrófagos, Anfípodos, Química
verde
AB Metal-organic frameworks (MOFs) are novel crystalline materials that are gaininga lot of attention in the environmental monitoring field. However, very little is knownabout the possible health or environmental risks that they may provoke. In the presentstudy, six MOFs, specifically UiO-64, UiO-66, CIM-80, CIM-81, CIM-82 and CIM-84,were synthesized and characterized. Two of them, UiO-64 and UiO-66 are MOFs widelyused; the other MOFs, with the abbreviations CIM (Canary Islands Materials), have beencharacterized in the University of La Laguna, and CIM-82 and CIM-84 are still in thephase of solving their crystalline structure. The cytotoxicity of these molecules wasevaluated in vitro with murine macrophages J774.A1 cell line, using the alamarBlue®method to obtain the lethal concentration that reduces 50% of these cells (CC50).Furthermore, it was evaluated their cytotoxicity in vivo using amphipod (Gammaropisatlantica). CIM-81 and CIM-82 were toxic in macrophages (CC50 of 0,98 ± 0,08 mg/mLand 0,88 ± 0,01 mg/mL, respectively) and in amphipods (all the individuals were dead).Therefore, there is a strong correlation between their in vivo and in vitro toxicity results.Two MOFs (UiO-64 and UiO-66) were more toxic to amphipods that to macrophages.CIM-80 and CIM-84 were not toxic.
LK http://riull.ull.es/xmlui/handle/915/16047
UL http://riull.ull.es/xmlui/handle/915/16047
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 27-abr-2024