RT info:eu-repo/semantics/bachelorThesis
T1 Peroxidasas del género Artemisia. Inducción y detección de actividad peroxidasa excretada en raíces "in vivo".
A1 Méndez Perdomo, Itahisa
K1 Artemisia thuscula, Guaiacol peroxidasa, Ascorbato peroxidasa
AB The genus Artemisia contains an important number of species that shownsecondary compounds with demonstrated biotechnological and pharmaceuticalinterest as the terpenoids artemisinin of A. annua. Different forms of cytoplasmicperoxidases enzymes are involved in the synthesis and degradation pathways;in a balance regulated both by external factors and by metabolic routes bellowingto growth and maturation. The principal task of this work is to detect in roots ofArtemisia thuscula, the peroxidase activity both kinetic and histological inconditions of induction of the secondary metabolism. Under our experimentalconditions, we have developed protocols for the detection and quantification ofthe enzymes guaiacol peroxidase and ascorbate peroxidase byspectrophotometric assay from root extracts of control plants and from inducedplants generated by the exposure to 285 nm UVB light. In addition, we havedeveloped protocols for the detection in root slices of the products generated byboth enzymes. Our results show the relative increase of ascorbate peroxidaseactivity and the decrease in guaiacol peroxidase activity in plants induced by UVBlight compared to controls. These results are consistent with the acceptedconcept in reference to the induction of secondary metabolism in plants, that typeI and III peroxidases respectively play.
YR 2019
FD 2019
LK http://riull.ull.es/xmlui/handle/915/16049
UL http://riull.ull.es/xmlui/handle/915/16049
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 27-dic-2024