RT info:eu-repo/semantics/bachelorThesis
T1 Elaboración de nanopartículas híbridas para la vectorización activa de oligonucleótidos.
A1 Santos Fagundo, Andrea
K1 Nanotecnología, Oligonucleótidos
AB The discovery of genes involved in the regulation of bone regeneration together with theprogress of nanotechnology, can led to the development of gene therapy systems with specific vectorization to cellular targets using molecules such as aptamers or antibodies.  In this work, the main objective was to obtain hybrid nanoparticles functionalized with an aptamer by surface adsorption able to present suitable physicochemical characteristics for their loading with antisense oligonucleotides for bone regenerative medicine. In this way, the influence of nanoparticles surface charge and aptamer- nanoparticles incubation conditions (ambient temperature for 1 hour, or 4ºC for 24 hours) on theadsorption efficiency of the aptamer was studied. Also, the modifications this adsorption produces on nanoparticles properties, specifically, size and Z potential was assessed. As result, an increase in the adsorption efficiency of the aptamer was observed with an increase in the positive surface charge of the nanoparticles, as well as an increase in the diameter and decrease of the Z potential at higher adsorption efficiency of the aptamer. No differences were observed in the adsorption efficiencies between both incubation conditions.
YR 2020
FD 2020
LK http://riull.ull.es/xmlui/handle/915/19989
UL http://riull.ull.es/xmlui/handle/915/19989
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 12-sep-2024