RT info:eu-repo/semantics/bachelorThesis T1 Nanopartículas híbridas para administración local A1 Gálvez Rodríguez, Sara K1 Terapia génica, Nanopartículas. AB Gene therapy shows promising outcomes for the local treatment of several tumors asuterine fibroids. Therefore, novel new non-viral gene vectors have been developedrecently, including nanoparticles. This work aims to analyze the influence ofnanoparticles surface components on their physicochemical characteristics. Thesecharacteristics should be controlled to allow subsequent functionalization processes forthe vectorization of oligonucleotides towards tumor cells.Hybrid nanoparticles were prepared using a modified nanoprecipitation technique.Experimentally, we have proved the modification of lipid components ratio(DOTAP:lecithin) affected nanoparticles Z potential. However, diameter andpolydispersity index remain unchanged, obtaining acceptable values for all theformulations tested.Finally, based on a bibliographic review of the strategies currently used to functionalizenanoparticles with antibodies and considering the key parameters that condition them, aprotocol was proposed to functionalize the nanoparticles with anti-CD24 antibody. Thisantibody has been chosen because CD24 protein is overexpressed on uterine fibroidscells surface. Thus, these functionalized nanoparticles could be used foroligonucleotides specific vectorization to tumor cells present on uterine fibroids. YR 2020 FD 2020 LK http://riull.ull.es/xmlui/handle/915/20472 UL http://riull.ull.es/xmlui/handle/915/20472 LA es DS Repositorio institucional de la Universidad de La Laguna RD 29-mar-2024