RT info:eu-repo/semantics/article
T1 Use of a pH-sensitive polymer in a microextraction and preconcentration method directly combined with high-performance liquid chromatography
A1 González-Martín, Raúl
A1 Pacheco-Fernández, Idaira
A1 Maiti, Binoy
A1 Ayala, Juan H.
A1 Afonso Perera, Ana María
A1 Díaz Díaz, David
A1 Pino, Verónica
K1 pH-sensitive polymer
K1 Hydrogel
K1 Microextraction
K1 Liquid chromatography
K1 Polycyclic aromatic hydrocarbons
K1 Urine
K1 polímeros sensibles al pH
K1 hidrogel
K1 microextracción
K1 cromatografía líquida
K1 hidrocarburos aromáticos policíclicos
K1 orina
AB A pH-sensitive polymer based on the poly(styrene- alt -maleic anhydride) co-polymer serves as basis to develop a microextraction method (pH-HGME) in direct combination with high-performance liquid chromatography (HPLC) and fluorescence detection (FD) for the determination of seven organic com- pounds, including three polycyclic aromatic hydrocarbons (PAHs), three monohydroxylated PAHs and one alkylphenol, in urine. The method bases on the structural modification of the pH-sensitive polymer in the aqueous sample at a high pH value, followed by the formation and insolubilization of a hydrogel contain- ing the preconcentrated analytes by decreasing the pH, and the direct injection of the hydrogel-rich phase in the HPLC-FD system. The optimization of the main variables permitted the selection of low amounts of aqueous sample (10 mL), which was mixed with 10 mg of co-polymer also present in a low volume (150 μL) of concentrated NaOH. The method further requires the addition of 200 μL of concentrated HCl, 3 min of stirring, and 15 min of centrifugation. This pH-HGME-HPLC-FD method presented low limits of detection, ranging from 0.001 μg L −1 to 0.09 μg L −1 in ultrapure water, average relative recoveries of 96.9% for the concentration level of 0.60 μg L −1 , and enrichment factors between 1.50 and 17.7. The pro- posed method also exhibited high precision, with intermediate relative standard deviations lower than 16% for a concentration level of 0.60 μg L −1 . The developed pH-HGME-HPLC-FD method performed ade- quately when analyzing two human urine samples provided by a non-smoker male and a smoker female, respectively. One of the target analytes (2-hydroxynaphthalene) was quantified in both samples using the standard addition method, with a predicted concentration of 7.3 ±0.4 μg L −1 in the non-smoker male urine and 19.3 ±0.6 μg L −1 in the smoker female urine.
PB Elsevier
YR 2020
FD 2020
LK http://riull.ull.es/xmlui/handle/915/21432
UL http://riull.ull.es/xmlui/handle/915/21432
LA en
NO Agencia Canaria de Investigación, Innovación y Sociedad de la Información
DS Repositorio institucional de la Universidad de La Laguna
RD 27-nov-2024