RT info:eu-repo/semantics/masterThesis
T1 Evaluación de actividad amebicida de productos marinos
A1 Molina Solano, Emmanuel David
A2 Máster Univ. en Investigación y Diagnóstico de Enfermedades Tropicales
AB In this study, the in vitro amoebicidal effect of 10 Sesquiterpenes extracted from Palythoa aff.clavata was evaluated against three different Acanthamoeba strains using a colorimetric assaybased on the alamarBlue®. Moreover, cytotoxicity studies in murine macrophages were carriedout using the same colorimetric test. In order to evaluate the action mode to induce cell death,different apoptosis elucidation kits were used.Our results showed that the tested sesquiterpenes presented in vitro anti-Acanthamoeba activityat low doses causing low toxicity to macrophages. Furthermore, guaititol 7 induced chromatincondensation, reduction of the ATP levels, mitochondrial membrane potential disruption andplasmatic membrane increased permeability in A. castellanii Neff, which indicate an activation ofa programmed cell death pathway. Thus, these molecules could serve as a potential alternativein therapy against Acanthamoeba infections.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/23083
UL http://riull.ull.es/xmlui/handle/915/23083
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 19-abr-2024