RT info:eu-repo/semantics/masterThesis
T1 Induction of antimicrobial activity in invertebrates
A1 González Acosta, Sergio
A2 Máster Universitario en Biomedicina Por la Universidad de la Laguna
AB Using antimicrobial peptides (AMPs) as an alternative toantimicrobials, has become an interesting approximation due the generationof antimicrobial resistance by microorganisms, which currently leads to aglobal health problem that will increase over time. Invertebrates represent arich niche for antimicrobial discovery due to their high diversity and thepresence of an adaptative immunity called “immune priming” describedduring the last decades. However, the lack of a standard model in the primingmethod makes studies on this field quite diverse. To carry out the inductionof antimicrobial activity in Tenebrio molitor, several priming methods(septic injury and oral administration) were tested with differentimmunogens (live and inactivated microorganisms) in order to optimize theexperimental design. Antimicrobial activity induction was demonstratedwith Escherichia coli through the screening assay, while the benefits of thispriming were not reflected in the survival assay. Due to the lack ofdetermining statistical significance, it is desirable to repeat the survival assaywith a larger sample size. Nevertheless, the fact that the methodology andthe demonstration of priming can be tested, means that this preliminary workserves as a basis for future researches in this field.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/23151
UL http://riull.ull.es/xmlui/handle/915/23151
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 27-jul-2024