RT info:eu-repo/semantics/masterThesis
T1 Development of phenotypic screening method based on cell staining
A1 Estupiñán Díaz, Ana Vaitiare
A2 Máster Universitario en Biomedicina
AB The traditional drug discovery process continues to be tedious, costly, time consuming and very risky. One of the hurdles that science faces is that reproducibility varies highly, or in some cases, the experimental results are non-reproducible at all. This drawback is even worse when considering drug development. Our research focuses on drug development evaluation at the early stages. We seek to revalue the traditional protocols in order to enrich our studies. Our hypothesis is that tissue-staining protocols can be adapted to monolayer cell culture to assess early pharmacological profiling of investigational compounds. The general objective is to adapt tissue-staining protocols to assess the early pharmacological profiling of investigational compounds. Some staining techniques such as eosin B, erythrosine and hematoxylin, alone or in combination with eosin B/ erythrosine, have been studied in previous works. In this work we will also explore the potential of other staining methods of interest, such as Sudan dyes for the visualization of lipids. In this work, we will explore the application of some of the existing cell and tissue staining protocols to our phenotypic drug discovery program directed at the discovery of small molecules for cancer treatment.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/23223
UL http://riull.ull.es/xmlui/handle/915/23223
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 25-abr-2024