RT info:eu-repo/semantics/masterThesis
T1 The role of Fanconi`s anaemia FANCM/Mph1 on chromosome segregation in Saccharomyces cerevisiae.
A1 Grzeszczuk, Joanna
A2 Máster Universitario en Biomedicina Por la Universidad de la Laguna
AB Inactivating mutations in the FANCM gene is one of the genetic causes of the Fanconi’sanaemia cancer-prone syndrome. Mph1 is the Saccharomyces cerevisiae ortholog forFANCM. This yeast has served as a model organism to understand the molecular function ofMph1. Thus, Mph1 is a component of the homologous recombination repair pathway (HR)that removes D-loops before they turn into Holliday junctions (HJs). In this way, cells favourrepair of several types of damage to the DNA while minimizing putative geneticrearrangements.Both D-loops and HJs are HR intermediates that hold sister chromatids together and need tobe processed before cells enter anaphase. In this work, we have looked at chromosomesegregation defects in anaphase cells of mph1 knock out (Δ) mutants. We have used singlemph1Δ mutants as well as double and triple mutant combinations with members of the HRpathway, either before (e.g., rad51Δ and rad54Δ) or after (mms4Δ and yen1Δ) the formationof the D-loop. We have found that cell cycle and chromosome segregation defects in mph1Δare surprisingly worse than rad51Δ/rad54Δ, at least for the DNA damage exerted by methylmethanesulphonate (MMS)
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/23671
UL http://riull.ull.es/xmlui/handle/915/23671
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 26-dic-2024