RT info:eu-repo/semantics/masterThesis
T1 Possible feedback mechanism between corticosteroids receptors and SGK1
A1 Martín Zurita, Miguel Eduardo
A2 Máster Universitario en Biomedicina Por la Universidad de la Laguna
AB Mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) are two nuclearreceptors that regulate transcription when they bind to their preferred corticosteroidhormone. The regulation of MR and GR have been intensely studied since they areinvolved in key physiological tasks including modulating ion and water transport or inenergetic homeostasis. To this day we are still discovering new functions of bothreceptors which have been shown to overlap or even interact between them. In this workwe propose that the serum and glucocorticoid-regulated kinase (SGK1) an MR and GRtarget activated by the phosphoinositide 3-linase pathway (PI3K) may regulate MR andGR function, providing a feedback loop in corticosteroid receptor signalling. To answerour hypothesis, we performed MR and GR dependent transactivation assays using fireflyluciferase as a reporter in N2a SGK1 knockout cells and HEK293T cells transfected withtwo SGK1 mutants, S422D (constitutively active) and K127A (catalytically inactive). Inaddition, we also studied the phosphorylating activity of the enzyme using one of itsknown target as a readout, the neural precursor cell expressed developmentally-downregulated 4-like (NEDD4-2).
YR 2022
FD 2022
LK http://riull.ull.es/xmlui/handle/915/28225
UL http://riull.ull.es/xmlui/handle/915/28225
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 26-abr-2024