RT info:eu-repo/semantics/masterThesis
T1 Early pharmacological profilling of bioactive natural products and their analogues
A1 González Bakker, Aday
A2 Máster Universitario en Biomedicina Por la Universidad de la Laguna
AB Bioprospection has a very important role in the discovery of compounds with antitumoractivity. A large number of the actual anticancer drugs has a natural origin. Due to the highbiodiversity around the world, which leads to new chemical structures (with new targets) andthe general multi-faceted mechanisms, makes necessary the use of phenotypic profiling todiscover and define the target of these compounds. Phenotypic drug discovery requires theuse of different assays to narrow down the possible mechanism of action such as theevaluation of cell migration and phenotypic changes in the cell. In this study, we carried outan optimization of in vitro wound healing assay and tested a panel of natural extracts ofHypericum genus to evaluate cell migration. We determined that in lung cancer cells A549,a serum concentration of 1% is suitable to evaluate cell migration. We observed differentialeffect of the extracts on migration with a considerable inhibition in cell migration by thehydroalcoholic extract of H. grandifolium. In addition, we designed a preliminary modelbased on Fourier’s Transform to use Nanolive’s CX-A imaging and analysis system for targetprediction. The designed model was able to preliminary cluster microtubule affecting drugswith similar mechanism of action to those of vinblastine and colchicine.
YR 2022
FD 2022
LK http://riull.ull.es/xmlui/handle/915/31997
UL http://riull.ull.es/xmlui/handle/915/31997
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 02-ene-2025