RT info:eu-repo/semantics/doctoralThesis
T1 Estudio de la capacidad inmunoprofiláctica de proteínas recombinantes de Leishmania infantum en el modelo murino
A1 Hernández Santana, Yasmina Esther
K1 Vacunas
K1 Inmunología
K1 Biología molecular
K1 Parasitología molecular
AB In this PhD Thesis, four proteins that are over-expressed in the infective cellular phase of the parasite L. infantum (STPKA, STPK, PP2B and PABP3) were purified to immunize BALB/c mice in order to study their protective capacity against VL. Three proteins induced a strong humoral response in mice, rLiSTPKA, rLiSTPK and rLiPABP3, but not rLiPP2B. Furthermore, the protein rLiPABP3 was the only one that induced a significant decreased of the parasite load both in spleen and liver of mice before 2 months post-infection with the parasite. In order to determine the effects of the immunisation with rLiPABP3 on the immune system of these mice, gene expression of 106 immune system-related genes was determined in the spleen of control, immunised and infected mice. The expression levels were compared with the gene expression data from control groups. The results showed that, along the whole experiment, rLiPABP3 promotes the inhibition of the inflammatory immune response in the spleen of infected mice. Furthermore, it was not observed any clearly polarized adaptive immune response to a particular cell type. One month after infection, the previously immunized animals showed over-expression of Il2rb, what makes us think that rLiPABP3 could stimulate the presence of memory T cells.In more advanced stages of infection, although we cannot observe a clear differentiation of a specific population of effector T lymphocytes, down-regulation of the gene encoding TNF-alpha was observed. This finding, together with the up-regulation of gene Cxcr4 and the absence of changes in the expression of the gene Ccr7, drives us to think that immunization not only keeps the micro-architecture of the spleen, but also promotes the successful migration of DCs from the MZ to the PALS, allowing the correct presentation of antigens to T cells and their subsequent activation, generating a protective immune response.
YR 2016
FD 2016
LK http://riull.ull.es/xmlui/handle/915/3325
UL http://riull.ull.es/xmlui/handle/915/3325
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 22-nov-2024