RT info:eu-repo/semantics/bachelorThesis
T1 Aislamiento de microARNs en muestras biológicas: potenciales biomarcadores epigenéticos de la COVID-19
A1 Sirvent Blanco, Candela
A2 Grado En Farmacia
K1 MicroARNs
K1 Biomarcadores
K1 COVID-19
AB The SARS-CoV2 global pandemic had an impact on public health and has opened up new ways to study. In this context, microRNAs have emerged as potential biomarkers for disease detection and understanding pathogenesis. MicroRNAs are small molecules of genetic material with the ability to regulate gene expression. It has been shown that miRNAs may be altered in some diseases, including COVID-19. In the present study, miRNAs were isolated from saliva and serum samples of COVID-19 cases using a commercial kit. Then a real-time qPCR was performed to confirm the correct extraction of microRNAs by amplifying stable reference miRNAs. In addition, miRNAs profiles expression were analyzed in serum and saliva samples from patients with viral infection (17 subjects) and compared with a control group (9 subjects) without infection. The results showed an efficient extraction of microRNAs for all the serum samples analyzed and for the 70% of saliva. A differential expression of miR-1246, which has been associated with virus pathogenesis, in serum samples was observed between cases and controls. On the other hand, in saliva samples, a significant overexpression of miR3195, was observed in COVID-19 cases. In conclusion, miRNAs are efficiently isolated from serum, while an improvement in the collection method and isolation protocol must be made for their isolation in saliva samples.  Our preliminary results suggest miR-1246 (in serum) and miR-3195 (in saliva) as potential diagnostic biomarkers of COVID-19 suggesting it’s possible role in the development of the disease.
YR 2023
FD 2023
LK http://riull.ull.es/xmlui/handle/915/33280
UL http://riull.ull.es/xmlui/handle/915/33280
LA es
DS Repositorio institucional de la Universidad de La Laguna
RD 27-dic-2024