RT info:eu-repo/semantics/article
T1 An association between viral genes and human oncogenic alterations: The adenovirus E1A induces the Ewing tumor fusion transcript EWS-FLI1
A1 Barrios del Pino, Yvelise
A1 Sánchez Prieto, Ricardo
A1 De Álava, Enrique
A1 Palomino, Teresa
A1 Guinea, Juan
A1 Fernández, Víctor
A1 Cebrián, Silvia
A1 Lleonart, Matilde
A1 Cabello, Pablo
A1 Martín, Pilar
A1 San Román, Carlos
A1 Bornstein, Rafael
A1 Pardo, Javier
A1 Martínez, Ana
A1 Díaz-Espada, Fernando
A1 Y Cajal, Santiago Ramón
AB Malignant transformation of human cells requires the accumulation of multiple genetic alterations, such as the activation of oncogenes and loss of function of tumor suppressor genes or those related to genomic instability. Among the genetic alterations most frequently found in human tumors are chromosomal translocations that may result in the expression of chimeric products with transforming capability or are able to change the expression of oncogenes. We show here that the adenovirus early region 1A (EIA) gene can induce a specific human fusion transcript (EWS-FLI1) that is characteristic of Ewing tumors. This fusion transcript was detected by RT-PCR in normal human fibroblasts and keratinocytes after expression of the adenovirus E1A gene, as well as in human cell lines immortalized by adenoviruses. Cloning and sequencing of the RT-PCR product showed fusion points between EWS and FLI1 cDNA identical to those detected in Ewing tumors. In addition, we detected a chimeric protein by western blot analysis and immunoprecipitation and a t(11,22) by fluorescent in situ hybridization. This association between a single viral gene and a specific human fusion transcript indicates a direct link between viral genes and chromosome translocations, one of the hallmarks of many human tum
SN 1078-8956
YR 1999
FD 1999
LK http://riull.ull.es/xmlui/handle/915/34877
UL http://riull.ull.es/xmlui/handle/915/34877
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 06-ago-2024