RT info:eu-repo/semantics/article
T1 Active idiotypic vaccination in a patient with biclonal follicular lymphoma
A1 Barrios del Pino, Yvelise
A1 Plaza, Aresio
A1 Cabrera, Rafael
A1 Yáñez, Rosa
A1 Suárez, Eduardo
A1 Fernández, Manuel Nicolás
A1 Díaz-Espada, Fernando
K1 Biclonal lymphoma
K1 Idiotypic immunotherapy
K1 Tomour vaccines
AB Specific immunological responses to the idiotypic epitopes present in the surface immunoglobulin (Ig) of the clonal tumour population can be induced for active immunotherapy in patients with B-cell non-Hodgkin lymphoma (NHL). The clonality of the tumour cells should have important implications for the success of the implemented therapy. Here we report on the case of a patient enrolled in a protocol of active idiotypic immunotherapy in which previous cytofluorometric analysis showed a major IgM+, κp+ population in the tumoral cell suspensions. However, sequence analysis of both tumour sample and tumour-derived hybrids revealed the presence of two unrelated clones that used different VH and VK gene segments. It was possible to obtain hybridomas secreting these two different IgM, κp idiotypic proteins. The patient was immunised with a mixture of these two idiotypic Igs conjugated to keyhole limpet haemocyanin. Anti-idiotypic antibodies directed against both tumour-associated proteins were detected. This is the first case of anti-idiotypic therapy in a patient with a biclonal NHL. Our work calls attention to the question of clonality in the context of idiotypic vaccination in NHL patients.
SN 0340-7004
YR 2001
FD 2001
LK http://riull.ull.es/xmlui/handle/915/34902
UL http://riull.ull.es/xmlui/handle/915/34902
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 12-nov-2024