RT info:eu-repo/semantics/article
T1 The Beauty of Simplicity: Delayed-Type Hypersensitivity Reaction to Measure Cellular Immune Responses in RNA-SARS-Cov-2 Vaccinated Individuals
A1 Barrios del Pino, Yvelise
A1 Franco, Andrés
A1 Sánchez-Machín, Inmaculada
A1 Poza-Guedes, Paloma
A1 González-Pérez, Ruperto
A1 Matheu, Víctor
K1 SARS-CoV-2
K1 DTH
K1 Skintest
K1 Delayed-type hypersensitivity
K1 T-cell response
K1 COVID-19
K1 Cell response
K1 Humoral response
AB Background: Monitoring cellular immune responses elicited in vaccinated individuals is highly complicated. Methods: 28 individuals participated during the vaccination process with 12 BNT162b2 mRNA (Pfizer) vaccine. Specific anti-RBD IgG using a classic ELISA was performed in days 10 and 20 (after one dose of the vaccine) and on day 35 (after two vaccine doses) in serum samples of all participants. In parallel, DTH (delayed-type hypersensitivity) Skin Test using S protein was performed before (11/28) and after two doses (28/28) of the vaccine. Results: 6/28 individuals were considered positive for the specific anti-RBD IgG positive at day 10, whereas all 28 individuals were positive at day 20. Moreover, 28/28 individuals increased the OD ratios at day 36 (2 doses). DTH cutaneous test was performed on 11/28 participants at day 20 (1 dose) showing 8/11 a positive reaction at 12 h. DTH of all participants was performed on day 36 (2 doses), showing 28/28 positive reactions at 12 h. Conclusion: This report describes the first publication of the results obtained using an in vivo method, the classical DTH response to the Spike protein to assess T-cell immune responses in vaccinated individuals. This affordable and simple test would help to answer basic immunogenicity questions on large-scale population vaccine studies.
SN 2076-393X
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/34908
UL http://riull.ull.es/xmlui/handle/915/34908
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 14-oct-2024