RT info:eu-repo/semantics/article T1 Easy approach to detect cell immunity to COVID vaccines in common variable immunodeficiency patients. A1 Barrios del Pino, Yvelise A1 Franco, Andrés A1 Alava-Cruz, Cristina A1 Cuesta-Martín, Ricardo A1 Camara, Carmen A1 Matheu, Victor A2 Medicina InternaDermatología y Psiquiatría A2 Hospital Universitario de Canarias K1 Common Variable Immunodeficiency (CVID) K1 Immunodeficiency K1 rare disease K1 T-cell response K1 COVID vaccination K1 DTH K1 skin test K1 SARS-CoV-2 K1 antibody deficiency AB Background: Patients with primary antibody deficiencies, such as Common VariableImmunodeficiency (CVID), have some problems to assess immune response after coronavirusdisease (COVID) vaccination. Cutaneous delayed-type hypersensitivity (DTH) has the potentialto be used as a useful, simple, and cheaper tool to assess T-cell (T lymphocyte) function.Methods: Seventeen patients with CVID, a rare disease, received two doses of the mRNA-basedPfizer-BioNTech COVID-19 vaccine. Humoral Immune Response (HIR) was determined by measuring specific immunoglobulin G (IgG) antibodies, and Cellular Immune Response (CIR) wasevaluated using an ex vivo interferon-gamma release assay (IGRA) and in vivo by DTH skin test.Results: Two weeks after the second dose of the vaccine, 12 out of 17 CVID patients have highoptical density (OD) ratios of specific anti-spike protein (S) IgG whereas five patients werenegative or low. Ex vivo CIR was considered positive in 14 out of 17 S1-stimulated patients.Unspecific stimulation was positive in all 17 patients showing no T-cell defect. A positive DTHskin test was observed in 16 CVID patients. The only patient with negative DTH also had negative ex vivo CIR.Conclusions: The use of DTH to evaluate CIR was validated with an optimal correlation withthe ex vivo CIR. The CIR after vaccination in patients with antibody deficiencies seems to havehigh precision and more sensitivity to antibodies-based methods in CVID.Clinical Implications: There is a remarkable correlation between cutaneous DTH and ex vivoIGRA after COVID vaccination. A COVID-specific skin DTH test could be implemented in largepopulations. YR 2022 FD 2022 LK http://riull.ull.es/xmlui/handle/915/34912 UL http://riull.ull.es/xmlui/handle/915/34912 LA en DS Repositorio institucional de la Universidad de La Laguna RD 08-oct-2024