RT info:eu-repo/semantics/article
T1 Easy approach to detect cell immunity to COVID vaccines in common variable immunodeficiency patients.
A1 Barrios del Pino, Yvelise
A1 Franco, Andrés
A1 Alava-Cruz, Cristina
A1 Cuesta-Martín, Ricardo
A1 Camara, Carmen
A1 Matheu, Victor
A2 Medicina InternaDermatología y Psiquiatría
A2 Hospital Universitario de Canarias
K1 Common Variable Immunodeficiency (CVID)
K1 Immunodeficiency
K1 rare disease
K1 T-cell response
K1 COVID vaccination
K1 DTH
K1 skin test
K1 SARS-CoV-2
K1 antibody deficiency
AB Background: Patients with primary antibody deficiencies, such as Common VariableImmunodeficiency (CVID), have some problems to assess immune response after coronavirusdisease (COVID) vaccination. Cutaneous delayed-type hypersensitivity (DTH) has the potentialto be used as a useful, simple, and cheaper tool to assess T-cell (T lymphocyte) function.Methods: Seventeen patients with CVID, a rare disease, received two doses of the mRNA-basedPfizer-BioNTech COVID-19 vaccine. Humoral Immune Response (HIR) was determined by measuring specific immunoglobulin G (IgG) antibodies, and Cellular Immune Response (CIR) wasevaluated using an ex vivo interferon-gamma release assay (IGRA) and in vivo by DTH skin test.Results: Two weeks after the second dose of the vaccine, 12 out of 17 CVID patients have highoptical density (OD) ratios of specific anti-spike protein (S) IgG whereas five patients werenegative or low. Ex vivo CIR was considered positive in 14 out of 17 S1-stimulated patients.Unspecific stimulation was positive in all 17 patients showing no T-cell defect. A positive DTHskin test was observed in 16 CVID patients. The only patient with negative DTH also had negative ex vivo CIR.Conclusions: The use of DTH to evaluate CIR was validated with an optimal correlation withthe ex vivo CIR. The CIR after vaccination in patients with antibody deficiencies seems to havehigh precision and more sensitivity to antibodies-based methods in CVID.Clinical Implications: There is a remarkable correlation between cutaneous DTH and ex vivoIGRA after COVID vaccination. A COVID-specific skin DTH test could be implemented in largepopulations.
YR 2022
FD 2022
LK http://riull.ull.es/xmlui/handle/915/34912
UL http://riull.ull.es/xmlui/handle/915/34912
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DS Repositorio institucional de la Universidad de La Laguna
RD 25-nov-2024