RT info:eu-repo/semantics/article
T1 A novel application of delayed-type hipersensitivity reaction to measure cellular immune response in SARS-CoV-2 exposed individuals.
A1 Barrios del Pino, Yvelise
A1 Franco, Andrés
A1 Sánchez Machín, Inmaculada
A1 Poza Guedes, Paloma
A1 González Pérez, Ruperto
A1 Matheu, Victor
A2 Medicina InternaDermatología y Psiquiatría
A2 Hospital Universitario de Canarias
K1 SARS-cov2
K1 DTH
K1 Skin test
K1 Delayed-type hypersensitivity
K1 T-cell response
K1 COVID-19
K1 Cell response
K1 Humoral response
AB Objective: To understand the anti-virus adaptive immune response occurring during SARS-Cov-2 infection isnecessary to have methods to investigate cellular and humoral components. The goal of this study has been toinvestigate the utility of a specific spike-DTH test using a coronavirus recombinant protein in COVID-19 patients.Methods: DTH studies were performed by intradermal injection of a commercial recombinant spike protein fromSARS-CoV-2 along with conventional serology studies.Results: Fifty-one COVID-19 patients were studied showing 84,3% of concordance with spike-DTH and anti-RBDIgG. Spike-DTH was superior to identify seven more COVID-19 individuals. A high specificity was found with nopositive spike DTH reactions in the non-sick individuals. The skin test also showed more stable results over timewhile specific anti-RBD-IgG decreased gradually. Clinical severity groups also showed a progressive gradient oflarger positive spike-DTH.Conclusion: Specific spike DTH test seems to be an easy method to study cell immune response
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/34913
UL http://riull.ull.es/xmlui/handle/915/34913
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 26-jun-2024