RT info:eu-repo/semantics/article
T1 Length of the antibody heavy chain complementarity determining region 3 as a specificity-determining factor
A1 Barrios del Pino, Yvelise
A1 Jirholtz, Pernilla
A1 Ohlin, Mats
K1 Antibody
K1 Antigen
K1 Complementarity determining region
K1 Diversity
K1 Loop
K1 Specificity
AB Theantigenbindingsiteofanantibodyismadeupofresiduesresidinginsixhypervariableloopsof the heavyandlightchains.Inmostcasesseveraloralloftheseloopsarerequiredfortheestablishmentofthe antigen-bindingsurface.Fiveoftheseloopsdisplayalimiteddiversityinlengthandsequencewhilethethird complementaritydeterminingregion(CDR)oftheheavychainishighlydifferentbetweenantibodiesnot onlywithrespecttosequencebutalsowithrespecttolength.Itsextensivediversityisakeycomponentinthe establishmentofbindingsitesallowingfortherecognitionofessentiallyanyantigenbyhumoralimmunity. Therelative importanceof its sequencevs its lengthdiversity inthiscontext ishowever, notverywell established.Toinvestigatethismatterfurtherwehaveusedanapproachemployingcombinatorialantibody librariesandantigen-specificselectioninthesearchforCDRH3lengthandsequencediversitycompatible withagivenantigenspecificity,themajorantigenicdeterminantonthetumour-associatedantigenmucin-1. InthiswaywehavenowdefinedheavychainCDR3lengthasacriticalparameterinthecreationofan antigen-specificbindingsite.Wealsoproposethatthismayreflectadependenceofaparticularstructureof thishypervariable loop, themajorcarrierofdiversity inthebindingsite, forestablishmentofagiven specificity
SN 0952-3499
YR 2004
FD 2004
LK http://riull.ull.es/xmlui/handle/915/34914
UL http://riull.ull.es/xmlui/handle/915/34914
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 01-jul-2024