RT info:eu-repo/semantics/article
T1 Human zygote morphological indicators of higher rate of arrest at the first cleavage stage
A1 Rodríguez Díaz, Rubí Nieves
A1 Blanes Zamora, Raquel
A1 Vaca Sánchez, Rebeca
A1 González Pérez, Jonay
A1 Báez Quintana, Delia
A1 Alberto Bethencourt, José Carlos
K1 First cleavage stage
K1 Dark halo
K1 Light halo
K1 Morphological features
K1 Nucleolar precursor body alignment
K1 Zygote arrest
AB A little studied aspect of developmental arrest (DA) in ART is zygote arrest (ZA). Etiologically, blockage at the first cleavage stage includes molecular and chromosomal anomalies, some of which manifest morphologically. Given considerations on embryo culture, transfer and cryopreservation, optimal zygote selection is very important. The aim of this study was to ascertain whether zygote morphologicalfeatures were indicators of increased ZA. In this study we performed a prospective, observational study of 2105 zygotes obtained from consecutive patients who were undergoing IVF/ICSI treatment, of which 43 (2%) suffered ZA. Morphological features observed under the inverted microscope werequalitatively categorized: pronuclear size, nucleolar precursor bodies (NPB) alignment, light and dark halos, polar body placement and fragmentation observed at 16–18 h post-insemination. We compared these features in blocked versus cleaved zygotes at 48 h and found significant correlations (p < 0.05) between ZA and three features: the absence of a light halo (p = 0.001), the absence of a dark halo (p < 0.005), and non-aligned NPB (p < 0.05). We can say that certain morphological features are indicators of significantly increased zygote arrest. These findings may be of utility for optimal zygote selection andculture strategies, especially in countries under restrictive conditions.
PB Cambridge University Press
SN 1469-8730
YR 2010
FD 2010
LK http://riull.ull.es/xmlui/handle/915/34987
UL http://riull.ull.es/xmlui/handle/915/34987
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 18-ene-2025