RT info:eu-repo/semantics/article
T1 Embryo sHLA-G secretion is related to pregnancy rate
A1 Rodríguez Díaz, Rubí Nieves
A1 Blanes Zamora, R.
A1 Vaca Sánchez, R.
A1 González Pérez, J.
K1 Embryo cohort
K1 IVF/ICSI outcome
K1 Pregnancy
K1 SECRETOMICS
K1 Soluble HLA-G
K1 Embryo transfer
AB HLA-G expression has been detected in early preimplantation embryos and it has beenpostulated that a relationship between embryonic expression of this factor and successfulpregnancy may exist. Forty-six patients were prospectively selected from our centre ‘Unidad deReproducción Humana, Hospital Universitario de Canarias’ for conducting this study. In all cases,metaphase II (MII) oocytes were fertilized using intracytoplasmic sperm injection 2–4 h afterretrieval. Embryos were cultured individually in 20 µl droplets of G-1 medium (VitroLife) underoil at 37°C and a 6% CO2 environment. Fertilization was assessed at 18 h postinsemination and alloocytes fertilized were passed into a new culture plaque individually in 300 µl culture mediumuntil day 3 of culture. The culture medium was examined for the expression and secretion ofsHLA-G with a sandwich enzyme-linked immunosorbent assay kit (BioVendor, Heidelberg,Germany) according to the manufacturer’s instructions. We found statistical significance between higher levels of sHLA-G secretion and pregnancy rate. When both groups were compared there was no difference in embryo quality of transferred embryos, but a significant difference in the number of oocytes and the embryo quality of the cohort existed that was greater in the pregnantgroup. A standardized sHLA-G assay with a specifically defined range and standard units provides a non-invasive method to identify the most competent embryos for transfer.
SN 0967-1994
YR 2019
FD 2019
LK http://riull.ull.es/xmlui/handle/915/34992
UL http://riull.ull.es/xmlui/handle/915/34992
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 07-ago-2024