RT info:eu-repo/semantics/article
T1 Altered Expression of AQP1 and AQP4 in Brain Barriers and Cerebrospinal Fluid May Affect Cerebral Water Balance during Chronic Hypertension.
A1 González Gómez, Miriam
A1 González Marrero, Ibrahim
A1 Hernández Abad, Luis G.
A1 Soto Viera, María
A1 Carmona Calero, Emilia M.
A1 Castañeyra Ruiz, Leandro
A1 Castañeyra Perdomo, Agustín
A2 Ciencias Médicas Básicas
A2 Grupo de investigación: Neurobiología Humana.
Instituto de Tecnologías Biomédicas (ITB)
Instituto Universitario de Neurociencia (IUNE)
K1 aquaporins
K1 hypertension
K1 SHR
K1 hydrocephalus
K1 choroid plexus
K1 cerebrospinal fluid
K1 ependyma
AB Hypertension is the leading cause of cardiovascular affection and premature death worldwide. The spontaneously hypertensive rat (SHR) is the most common animal model of hypertension,which is characterized by secondary ventricular dilation and hydrocephalus. Aquaporin (AQP) 1 and4 are the main water channels responsible for the brain’s water balance. The present study focuseson defining the expression of AQPs through the time course of the development of spontaneouschronic hypertension. We performed immunofluorescence and ELISA to examine brain AQPs from10 SHR, and 10 Wistar–Kyoto (WKY) rats studied at 6 and 12 months old. There was a significantdecrease in AQP1 in the choroid plexus of the SHR-12-months group compared with the age-matchedcontrol (p < 0.05). In the ependyma, AQP4 was significantly decreased only in the SHR-12-monthsgroup compared with the control or SHR-6-months groups (p < 0.05). Per contra, AQP4 increased inastrocytes end-feet of 6 months and 12 months SHR rats (p < 0.05). CSF AQP detection was higherin the SHR-12-months group than in the age-matched control group. CSF findings were confirmedby Western blot. In SHR, ependymal and choroidal AQPs decreased over time, while CSF AQPslevels increased. In turn, astrocytes AQP4 increased in SHR rats. These AQP alterations may underliehypertensive-dependent ventriculomegaly
YR 2022
FD 2022
LK http://riull.ull.es/xmlui/handle/915/34998
UL http://riull.ull.es/xmlui/handle/915/34998
LA en
NO Int J Mol Sci. 2022 Oct 14;23(20):12277. doi: 10.3390/ijms232012277. PMID: 36293145; PMCID: PMC9603298.González-Marrero I, Hernández-Abad LG, González-Gómez M, Soto-Viera M, Carmona-Calero EM, Castañeyra-Ruiz L, Castañeyra-Perdomo A.
DS Repositorio institucional de la Universidad de La Laguna
RD 21-may-2024