RT info:eu-repo/semantics/article
T1 Ultrastructural study of platelet behavior and interrelationship in sprouting and intussusceptive angiogenesis during arterial intimal thickening formation.
A1 González Gómez, Miriam
A1 Díaz Flores, Lucio
A1 Gutiérrez García, Ricardo
A1 García, María Pino
A1 Díaz Flores, Lucio
AD  jr.
A1 Gayoso, Sara
A1 Carrasco Juan, José Luis
A1 Álvarez Argüelles, Hugo
K1 Platelets
K1 Intimal thickening
K1 Sprounting angiogenesis
K1 Vascular regression
AB Platelets in atherosclerosis, bypass stenosis, and restenosis have been extensively assessed.However, a sequential ultrastructural study of platelets in angiogenesis during the early phases ofthese lesions has received less attention. Our objective was the study of platelets in angiogenesis andvessel regression during intimal thickening (IT) formation, a precursor process of these occlusivevascular diseases. For this purpose, we used an experimental model of rat occluded arteries andprocedures for ultrastructural observation. The results show (a) the absence of platelet adhesionin the de-endothelialized occluded arterial segment isolated from the circulation, (b) that intraarterialmyriad platelets contributed from neovessels originated by sprouting angiogenesis from theperiarterial microvasculature, (c) the association of platelets with blood components (fibrin, neutrophils,macrophages, and eosinophils) and non-polarized endothelial cells (ECs) forming aggregates(spheroids) in the arterial lumen, (d) the establishment of peg-and-socket junctions between plateletsand polarized Ecs during intussusceptive angiogenesis originated from the EC aggregates, withthe initial formation of IT, and (e) the aggregation of platelets in regressing neovessels (‘transitoryparacrine organoid’) and IT increases. In conclusion, in sprouting and intussusceptive angiogenesisand vessel regression during IT formation, we contribute sequential ultrastructural findings onplatelet behavior and relationships, which can be the basis for further studies using other procedures.
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/35015
UL http://riull.ull.es/xmlui/handle/915/35015
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 21-may-2024