RT info:eu-repo/semantics/article
T1 Organotypic culture as a research and preclinical model to study uterine leiomyomas
A1 Salas, Ana
A1 López, Judith
A1 Reyes, Ricardo
A1 Évora, Carmen
A1 Montes de Oca, Francisco
A1 Báez Quintana, Delia Rosa
A1 Delgado, Araceli
A1 Almeida, Teresa A.
A2 
K1 
K1 Fibroma
K1 Útero
K1 Uterine leiomyomas
AB Organotypic cultures of tissue slices have been successfully established in lung, prostate, colon, gastric and breast cancer among other malignancies, but until now an ex vivo model based on tissue slices has not been established for uterine leiomyoma. In the present study, we describe a method for culturing tumour slides onto an alginate scafold. Morphological integrity of tissue slices was maintained for up to 7 days of culture, with cells expressing desmin, estrogen and progesterone receptors. Driver mutations were present in the ex vivo slices at all-time points analyzed. Cultivated tumour slices responded to ovarian hormones stimulation upregulating the expression of genes involved in leiomyoma pathogenesis. This tissue model preserves extracellular matrix, cellular diversity and genetic background simulating more in-vivo-like situations. As a novelty, this platform allows encapsulation of microspheres containing drugs that can be tested on the ex vivo tumour slices. After optimizing drug release rates, microspheres would then be directly tested in animal models through local injection.
YR 2020
FD 2020
LK http://riull.ull.es/xmlui/handle/915/35154
UL http://riull.ull.es/xmlui/handle/915/35154
LA 
NO 
DS Repositorio institucional de la Universidad de La Laguna
RD 24-nov-2024