RT info:eu-repo/semantics/article
T1 Dual agonist-antagonist effect of ulipristal acetate in human endometrium and myometrium
A1 Báez Quintana, Delia Rosa
A1 Salas, Ana
A1 Vázquez, Paula
A1 Bello, Aixa R.
A1 Almeida, Teresa A.
K1 Endometrium
K1 Fibroids
K1 Myometrium
K1 NanoString platform
K1 qPCR
K1 Ulipristal acetate
K1 Fibrosis
AB The aim of this study was to assess the molecular effect of ulipristal acetate (UPA) on gene expression in myometrium and endometrium of patients with symptomatic fibroids. Tissues isolated from fourwomen treated preoperatively with UPA (5 mg) were compared to those from untreated controlsusing NanoString platform to assess the expression of 75 candidate genes modulated by UPA andovarian steroids. Deregulated genes were then validated by real-time PCR. In myometrium, UPA exerted an antagonistic effect similar to that observed in fibroids. In UPA-treated endometrium, six genes were identified as highly and significantly upregulated, including matricellular genes CCN1 (54-fold, P = 0.0018) and CCN2 (11-fold, P = 0.00044), Krüppel-like factor 4 (>3-fold, P = 0.0036), and mast cell markers including tryptases TPSAB1/TPSB2 (31-fold, P = 0.023) and carboxypeptidase A (CPA3, 17-fold, P = 0.05). In endometrium, UPA induced the expression of genes involved in fibrogenesis and mast cellfunction—some of them being widely involved in hepatic injury, which could explain the markedfibrosis and inflammatory cell infiltration observed in explanted livers from patients under UPAtreatment.
SN 1744-8352
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/35155
UL http://riull.ull.es/xmlui/handle/915/35155
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 21-dic-2024