RT info:eu-repo/semantics/article
T1 Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse
A1 Rodríguez Bello, Aixa Celina
A1 Puertas Avendaño, R. A.
A1 González Gómez, M. J.
A1 González Gómez, M.
A1 Laborda, J.
A1 Damas, C.
A1 Ruiz Hidalgo, M.
A1 Diaz, C.
A2 BioquímicaMicrobiología, Biología Celular y Genética
A2 Instituto de Enfermedades  tropicales y salud pública
K1 anterior pituitary
K1 hormone receptors
K1 leptin
K1 leptin receptor
AB With the aim of studying delta-like protein 1 (DLK1) with respect to the relationshipbetween adipocyte leptin and adenohypophyseal hormones, we carried out an immunohistochemical study analysing the presence of receptors for these hormones in thepituitary and adipose cells of male wild-type (WT) mice (Dlk1+/+) compared to knockout (KO) mice (Dlk1−/−). The mRNA expression of these molecules was also determined using the reverse transcriptase-polymerase chain reaction. The results obtainedshowed that, in WT adipose cells, all of the adenohypophyseal hormone receptorswere present, with a higher mRNA expression for growth hormone (GH) receptor andthyroid-stimulating hormone (TSH) receptor. Of the total cells in the anterior pituitarylobe, 17.09±0.9% were leptin receptor (LEPR) immunoreactive (-IR), mainly in GH-IRand prolactin (PRL)-IR cells (41.5±3.8%; 13.5±1.7%, respectively). In Dlk1−/− mice, adipocyte cells showed a significant increase in the TSH receptor mRNA expression level.Moreover, the percentage of LEPR-IR GH cells showed a statistically significant increase compared to controls, from 41.5±3.8% to 53.1±4.0%. By contrast, only3.0±0.6% of LEP-IR anterior pituitary cells were detected in Dlk1 KO mice, as opposedto 6.8±1.1% observed in WT mice. The results suggest that relationships exist between adipocytes and pituitary GH, PRL and TSH cells, in addition to an influence withrespect to the synthesis and release of pituitary leptin, particularly in PRL cells.
YR 2016
FD 2016
LK http://riull.ull.es/xmlui/handle/915/35281
UL http://riull.ull.es/xmlui/handle/915/35281
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 27-nov-2024