RT info:eu-repo/semantics/article
T1 Expression of tachykinins and tachykinin receptors and Interaction with Kisspeptin in
human granulosa and cumulus cells1
A1 Rodríguez Bello, Aixa Celina
A1 García Ortega, Jordán
A1 Pinto, Francisco M.
A1 Prados, Nicolás
A1 Almeida, Teresa A.
A1 Ferna´ndez-Sa´nchez, Manuel
A1 Candenas, Luz
A2 BioquímicaMicrobiología, Biología Celular y Genética
A2 Instituto de enfermedades tropicales y Salud Pu¿blica de CanariasUniversidad de La Laguna
K1 hemokinin-1
K1 human cumulus cells
K1 human granulosa cells
K1 kisspeptin
K1 substance P
K1 tachykinin NK1 receptor
K1 tachykinin NK2 receptor
AB The neurokinin B/NK3 receptor (NK3R) and kisspeptin/kisspeptin receptor (KISS1R), two systems which are essentialfor reproduction, are coexpressed in human mural granulosa(MGC) and cumulus cells (CCs). However, little is known aboutthe presence of other members of the tachykinin family in thehuman ovary. In the present study, we analyzed the expressionof substance P (SP), hemokinin-1 (HK-1), NK1 receptor (NK1R),and NK2 receptor (NK2R) in MGCs and CCs collected frompreovulatory follicles of oocyte donors at the time of oocyteretrieval. RT-PCR, quantitative RT-PCR, immunocytochemistry,and Western blotting were used to investigate the patterns ofexpression of tachykinin and tachykinin receptor mRNAs andproteins and the possible interaction between the tachykininfamily and kisspeptin. Intracellular free Ca2+ levels ([Ca2+]i) inMGCs after exposure to SP or kisspeptin in the presence of SPwere also measured. We found that SP, HK-1, the truncatedNK1R isoform NK1R-Tr, and NK2R were all expressed in MGCsand CCs. NK1R-Tr mRNA and NK2R mRNA and protein levelswere higher in MGCs than in CCs from the same patients.Treatment of cells with kisspeptin modulated the expression ofHK-1, NK3R, and KISS1R mRNAs, whereas treatment with SPregulated kisspeptin mRNA levels and reduced the [Ca2+]iresponse produced by kisspeptin. These data demonstrate thatthe whole tachykinin system is expressed and acts in coordination with kisspeptin to regulate granulosa cell function in thehuman ovary.
YR 2016
FD 2016
LK http://riull.ull.es/xmlui/handle/915/35304
UL http://riull.ull.es/xmlui/handle/915/35304
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 27-nov-2024