RT nfo:eu-repo/semantics/article
T1 Altered expression of the
tachykinins substance P/neurokinin
A/hemokinin-1 and their preferred
neurokinin 1/neurokinin 2 receptors
in uterine leiomyomata
A1 Rodríguez Bello, Aixa Celina
A1 González Santana, Ayoze
A1 Marrero Hernandez, Sara
A1 Dorta, Idaira
A1 Hernández, Mariano
A1 Pinto, Francisco María
A1 Baez, Delia
A1 Candenas, Luz
A1 Almeida, Teresa A.
A2 BioquímicaMicrobiología, Biología Celular y Genética
A2 Instituto de enfermedades tropicales y salud pública de canariasUniversidad de la Laguna, Tenerife;
K1 Leiomyomas
K1 myometrium
K1 NK1 receptor short isoform
K1 tachykinins
K1 tachykinin receptors
AB Objective: To study the expression levels of tachykinins and tachykinin receptors in uterine leiomyomas and matched myometrium.Design: Laboratory study.Setting: University research laboratories and academic hospital.Patient(s): Women undergoing hysterectomy for symptomatic leiomyomas.Intervention(s): Quantitative polymerase chain reaction, immunohistochemistry and Western blot.Main Outcome Measure(s): Expression and tissue immunostaining of substance P, neurokinin A, hemokinin-1, neurokinin 1 receptorfull-length (NK1R-Fl) and truncated (NK1R-Tr) isoforms, and neurokinin 2 receptor (NK2R) in paired samples of leiomyoma andadjacent normal myometrium.Result(s): TAC1 messenger RNA (mRNA) was significantly up-regulated in leiomyomas, whereas intense immunoreaction for the threepeptides was particularly abundant in connective tissue cells. Differential regulation of TACR1 mRNA was observed, and at the proteinlevel there was a significant increased expression of NK1R short isoform (NK1R-Tr). TACR2 mRNA was significantly up-regulated inleiomyomas, although levels of NK2R protein were similar in normal and tumor cells.Conclusion(s): These and our previous data demonstrate that the whole tachykinin system is differentially regulated in leiomyomas.The increased expression of NK1R-Tr might stimulate leiomyoma growth in a similar way to that observed in other steroid-dependenttumors. (Fertil Steril  2016;106:1521–9. 2016 by American Society for Reproductive Medicine.)
YR 2016
FD 2016
LK http://riull.ull.es/xmlui/handle/915/35305
UL http://riull.ull.es/xmlui/handle/915/35305
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 24-nov-2024