RT info:eu-repo/semantics/article
T1 MicroRNA Profile in CD8+ T-Lymphocytes from HIV-Infected Individuals: Relationship with Antiviral Immune Response and Disease Progression.
A1 Valenzuela Fernández, Agustín
A1 Egaña Gorroño, Lander
A1 C. Guardo, Alberto
A1 Bargalló, Manel E.
A1 Planet, Evarist
A1 Vilaplana, Elisenda
A1 Escribà, Tuixent
A1 Pérez, Iñaki
A1 Gatell, Josep Maria
A1 García, Felipe
A1 Arnedo, Mireia
A1 Plana M., Montserrat
A1 HIV Controllers Consortium of the AIDS Spanish
Network
A2 Medicina Física y Farmacología
A2 Grupo "Inmunología Celular y Viral".
AB BackgroundThe relationship between host microRNAs (miRNA), viral control and immune responsehas not yet been elucidated in the field of HIV. The aim of this study was to assess the differential miRNA profile in CD8+ T-cells between HIV-infected individuals who differ in terms ofviral replication control and immune response.MethodsmiRNA profile from resting and CD3/CD28-stimulated CD8+ T-cells from uninfected individuals (HIV-, n = 11), Elite Controllers (EC, n = 15), Viremic Controllers (VC, n = 15), ViremicProgressors (VP, n = 13) and HIV-infected patients on therapy (ART, n = 14) was assessedusing Affymetrix miRNA 3.1 arrays. After background correction, quantile normalization andmedian polish summarization, normalized data were fit to a linear model. The analysis comprised: resting samples between groups; stimulated samples between groups; and stimulated versus resting samples within each group. Enrichment analyses of the putative targetgenes were perfomed using bioinformatic algorithms..ResultsA downregulated miRNA pattern was observed when resting samples from all infectedgroups were compared to HIV-. A miRNA downregulation was also observed when stimulated samples from EC, ART and HIV- groups were compared to VP, being hsa-miR-4492the most downregulated. Although a preferential miRNA downregulation was observedwhen stimulated samples were compared to the respective resting samples, VP presenteda differential miRNA expression pattern. In fact, hsa-miR-155 and hsa-miR-181a weredownregulated in VP whereas in the other groups, either an upregulation or no differenceswere observed after stimulation, respectively. Overall, functional enrichment analysisrevealed that the predicted target genes were involved in signal transduction pathways,metabolic regulation, apoptosis, and immune response.ConclusionsResting CD8+ T-cells do not exhibit a differential miRNA expression between HIV-infectedindividuals but they do differ from non-infected individuals. Moreover, a specific miRNA pattern is present in stimulated CD8+ T-cells from VP which could reflect a detrimental patternin terms of CD8+ T-cell immune response.
YR 2016
FD 2016
LK http://riull.ull.es/xmlui/handle/915/35318
UL http://riull.ull.es/xmlui/handle/915/35318
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 08-nov-2024