RT info:eu-repo/semantics/article
T1 Bioinformatic approaches to draft the viral genome sequence of Canary Islands cases related to the multicountry mpox virus 2022-outbreak.
A1 Valenzuela Fernández, Agustín
A1 Muñoz Barrera, Adrián
A1 Ciuffreda, Laura
A1 Alcoba Florez, Julia
A1 Rubio Rodríguez, Luis A.
A1 Rodríguez Pérez, Héctor
A1 Gil Campesino, Helena
A1 García Martínez de Artola, Diego
A1 Salas Hernández, Josmar
A1 Rodríguez Núñez, Julia
A1 Íñigo Campos , Antonio
A1 García Olivares, Víctor
A1 Díez Gil, Oscar
A1 González Montelongo, Rafaela
A1 Lorenzo Salazar, José M.
A1 Flores, Carlos
K1 Bioinformatics
K1 Viral surveillance
K1 Genome assembly
AB On July 23, 2022, monkeypox disease (mpox) was declared a Public Emergency of International Concern(PHEIC) by the World Health Organization (WHO) due to a multicountry outbreak. In Europe, several casesof mpox virus (MPXV) infection related to this outbreak were detected in the Canary Islands (Spain). Herewe describe the combination of viral DNA sequencing and bioinformatic approaches, including methods forde novo genome assembly and short- and long-read technologies, used to reconstruct the first MPXVgenome isolated in the Canary Islands on the 31st of May 2022 from a male adult patient with mildsymptoms. The same sequencing and bioinformatic approaches were then validated with three other positive cases of MPXV infection from the same mpox outbreak. We obtained the best results using a reference-based approach with short reads, evidencing 46–79 nucleotide variants against viral sequencesfrom the 2018–2019 mpox outbreak and placing the viral sequences in the new B.1 sublineage of clade IIb ofthe MPXV classification. This study of MPXV demonstrates the potential of metagenomics sequencing forrapid and precise pathogen identification.
SN 2001-0370
YR 2023
FD 2023
LK http://riull.ull.es/xmlui/handle/915/35320
UL http://riull.ull.es/xmlui/handle/915/35320
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 26-dic-2024