RT info:eu-repo/semantics/article
T1 Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection.
A1 Valenzuela Fernández, Agustín
A1 Alcoba Florez, Julia
A1 Gil-Campesino, H.
A1 Artola, D.G.-M.D.
A1 González-Montelongo, R.
A1 Ciuffreda, L.
A1 Flores, C.
K1 COVID-19
K1 SARS-CoV-2
K1 Diagnosis
K1 False negatives
K1 Solution comparisons
K1 Sensitivity
AB Objectives: The ongoing COVID-19 pandemic continues to impose demands on diagnostic screening. In anticipation that the recurrence of outbreaks and the measures for lifting the lockdown worldwide may cause supply chain issues over the coming months, this study assessed the sensitivity of a number of one-step retrotranscription and quantitative polymerase chain reaction (RT-qPCR) solutions to detect SARS-CoV-2. Methods: Six different RT-qPCR alternatives were evaluated for SARS-CoV-2/COVID-19 diagnosis based on standard RNA extractions. The one with best sensitivity was also assessed with direct nasopharyngeal swab viral transmission medium (VTM) heating; thus overcoming the RNA extraction step. Results: A wide variability in the sensitivity of RT-qPCR solutions was found that was associated with a range of false negatives from 2% (0.3–7.9%) to 39.8% (30.2–50.2%). Direct preheating of VTM combined with the best solution provided a sensitivity of 72.5% (62.5–81.0%), in the range of some of the solutions based on standard RNA extractions. Conclusions: Sensitivity limitations of currently used RT-qPCR solutions were found. These results will help to calibrate the impact of false negative diagnoses of COVID-19, and to detect and control new SARS-CoV-2 outbreaks and community transmissions.
SN 1878-3511
YR 2020
FD 2020
LK http://riull.ull.es/xmlui/handle/915/35330
UL http://riull.ull.es/xmlui/handle/915/35330
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 14-oct-2024