RT info:eu-repo/semantics/article
T1 Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples.
A1 Valenzuela Fernández, Agustín
A1 Alcoba Florez, Julia
A1 González Montelongo, Rafaela
A1 Íñigo Campos, Antonio
A1 García Martínez de Artola, Diego
A1 Gil Campesino, Helena
A1 The Microbiology Technical Support Team
A1 Ciuffreda, Laura
A1 Flores, Carlos
A2 Medicina Física y Farmacología
A2 Grupo "Inmunología Celular y Viral".
K1 COVID-19
K1 SARS-CoV-2
K1 diagnosis
K1 sample treatment
K1 RNA extraction
K1 fast protocols
AB The current reference for COVID-19 diagnosis is based on the detection of SARS-CoV-2 on RNA extracts usingone-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughputCOVID-19 screening, we tested the performance of three alternative, simple and affordable protocols torapidly detect SARS-CoV-2, overcoming the long and tedious RNA extraction step. Although with an averageincrease of 6.1 (± 1.6) cycles compared to standard tests with RNA extracts, we show that RT-qPCR yieldedconsistent results in nasopharyngeal swab samples that were subject to a direct 70oC incubation for 10 min.Our findings provide viable options to overcome any supply chain issue and help to increase the throughputof diagnostic tests by using any qPCR device, thereby complementing standard COVID-19 testing.
YR 2020
FD 2020
LK http://riull.ull.es/xmlui/handle/915/35334
UL http://riull.ull.es/xmlui/handle/915/35334
LA en
NO medRxiv preprint doi: https://doi.org/10.1101/2020.04.08.20058495; this version posted April 11, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license .
DS Repositorio institucional de la Universidad de La Laguna
RD 12-may-2024