RT info:eu-repo/semantics/article
T1 Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection.
A1 Valenzuela Fernández, Agustín
A1 Alcoba Florez, Julia
A1 Gil Campesino, Helena
A1 García Martínez de Artola, Diego
A1 González Montelongo, Rafaela
A1 Ciuffreda, Laura
A1 Flores, Carlos
K1 COVID-19
K1 SARS-CoV-2
AB Objectives: The ongoing COVID-19 pandemic continues to impose demands on diagnostic screening. Inanticipation that the recurrence of outbreaks and the measures for lifting the lockdown worldwide maycause supply chain issues over the coming months, this study assessed the sensitivity of a number of onestep retrotranscription and quantitative polymerase chain reaction (RT-qPCR) solutions to detect SARSCoV-2.Methods: Six different RT-qPCR alternatives were evaluated for SARS-CoV-2/COVID-19 diagnosis based onstandard RNA extractions. The one with best sensitivity was also assessed with direct nasopharyngealswab viral transmission medium (VTM) heating; thus overcoming the RNA extraction step.Results: A wide variability in the sensitivity of RT-qPCR solutions was found that was associated with arange of false negatives from 2% (0.3–7.9%) to 39.8% (30.2–50.2%). Direct preheating of VTM combinedwith the best solution provided a sensitivity of 72.5% (62.5–81.0%), in the range of some of the solutionsbased on standard RNA extractions.Conclusions: Sensitivity limitations of currently used RT-qPCR solutions were found. These results willhelp to calibrate the impact of false negative diagnoses of COVID-19, and to detect and control new SARSCoV-2 outbreaks and community transmissions.
SN 1878-3511
YR 2020
FD 2020
LK http://riull.ull.es/xmlui/handle/915/35338
UL http://riull.ull.es/xmlui/handle/915/35338
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 14-oct-2024