RT info:eu-repo/semantics/article
T1 A conserved uORF regulates APOBEC3G translation and is targeted by HIV-1 Vif protein to repress the antiviral factor
A1 Valenzuela Fernández, Agustín
A1 Libre, Camille
A1 Seissler, Tanja
A1 Guerrero, Santiago
A1 Batisse, Julien
A1 Verriez, Cédric
A1 Stupfler, Benjamin
A1 Gilmer, Orian
A1 Cabrera Rodriguez, Romina
A1 Weber, Melanie M.
A1 Cimarelli, Andrea
A1 Etienne, Lucie
A1 Marquet, Roland
A1 Paillart, Jean-Christophe
A2 Medicina Física y Farmacología
A2 Grupo "Inmunología Celular y Viral".
AB The HIV-1 Vif protein is essential for viral fitness and pathogenicity. Vif decreases expression ofcellular restriction factors APOBEC3G (A3G), A3F, A3D and A3H, which inhibit HIV-1 replication byinducing hypermutation during reverse transcription. Vif counteracts A3G at several levels(transcription, translation and protein degradation) that together reduce the levels of A3G in cells andprevent its incorporation into viral particles. How Vif affects A3G translation remains unclear. Here, weuncovered the importance of a short conserved uORF (upstream ORF) located within two criticalstem-loop structures of the 5’ untranslated region (5’UTR) of A3G mRNA for this process. A3Gtranslation occurs through a combination of leaky-scanning and translation re-initiation and thepresence of an intact uORF decreases the extent of global A3G translation under normal conditions.Interestingly, the uORF is also absolutely required for Vif-mediated translation inhibition andredirection of A3G mRNA into stress granules. Overall, we discovered that A3G translation isregulated by a small uORF conserved in the human population and that Vif uses this specific featureto repress its translation
YR 2021
FD 2021
LK http://riull.ull.es/xmlui/handle/915/35350
UL http://riull.ull.es/xmlui/handle/915/35350
LA en
NO bioRxiv preprint doi: https://doi.org/10.1101/2021.01.13.426487; this version posted October 8, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license.
DS Repositorio institucional de la Universidad de La Laguna
RD 23-may-2024