RT info:eu-repo/semantics/article
T1 Viral Characteristics Associated with the Clinical Nonprogressor Phenotype Are Inherited by Viruses from a Cluster of HIV-1 Elite Controllers.
A1 Valenzuela Fernández, Agustín
A1 Casado, C.
A1 Marrero-Hernández, S.
A1 Márquez-Arce, D.
A1 Pernas, M.
A1 Marfil, S.
A1 Borràs-Grañana, F.
A1 Olivares, I.
A1 Cabrera-Rodríguez, R.
A1 Valera, M.-S.
A1 de Armas-Rillo, L.
A1 Lemey, P.
A1 Blanco, J.
A1 Lopez-Galíndez, C.
K1 CD4binding
K1 HIV-1
K1 Heritability
K1 LTNP-EC
K1 Actin-tubulinmodifications
K1 Cell signaling
K1 Phylogenetic analysis
K1 Viral envelope
AB A small group of HIV-1-infected individuals, called long-term nonprogressors (LTNPs), and in particular a subgroup of LTNPs, elite controllers (LTNP-ECs), display permanent control of viral replication and lack of clinical progression. This control is the result of a complex interaction of host, immune, and viral factors. We identified, by phylogenetic analysis, a cluster of LTNP-ECs infected with very similar low-replication HIV-1 viruses, suggesting the contribution of common viral features to the clinical LTNP-EC phenotype. HIV-1 envelope (Env) glycoprotein mediates signaling and promotes HIV-1 fusion, entry, and infection, being a key factor of viral fitness in vitro, cytopathicity, and infection progression in vivo. Therefore, we isolated full-length env genes from viruses of these patients and from chronically infected control individuals. Functional characterization of the initial events of the viral infection showed that Envs from the LTNP-ECs were ineffective in the binding to CD4 and in the key triggering of actin/tubulin-cytoskeleton modifications compared to Envs from chronic patients. The viral properties of the cluster viruses result in a defective viral fusion, entry, and infection, and these properties were inherited by every virus of the cluster. Therefore, inefficient HIV-1 Env functions and signaling defects may contribute to the low viral replication capacity and transmissibility of the cluster viruses, suggesting a direct role in the LTNP-EC phenotype of these individuals. These results highlight the important role of viral characteristics in the LTNP-EC clinical phenotype. These Env viral properties were common to all the cluster viruses and thus support the heritability of the viral characteristics. IMPORTANCE HIV-1 long-term nonprogressor elite controller patients, due to their permanent control of viral replication, have been the object of numerous studies to identify the factors responsible for this clinical phenotype. In this work, we analyzed the viral characteristics of the envelopes of viruses from a phylogenetic cluster of LTNP-EC patients. These envelopes showed ineffective binding to CD4 and the subsequent signaling activity to modify actin/tubulin cytoskeletons, which result in low fusion and deficient entry and infection capacities. These Env viral characteristics could explain the nonprogressor clinical phenotype of these patients. In addition, these inefficient env viral properties were present in all viruses of the cluster, supporting the heritability of the viral phenotype.
SN 2150-7511
YR 2018
FD 2018
LK http://riull.ull.es/xmlui/handle/915/35351
UL http://riull.ull.es/xmlui/handle/915/35351
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 09-nov-2024