RT info:eu-repo/semantics/article
T1 HIV-1 Env associates with HLA-C free-chains at the cell membrane modulating viral infectivity.
A1 Valenzuela Fernández, Agustín
A1 Serena, Michela
A1 Parolini, Francesca
A1 Biswas, Priscilla
A1 Sironi, Francesca
A1 Blanco Miranda, Almudena
A1 Zoratti, Elisa
A1 Scupoli, Maria Teresa
A1 Ziglio, Serena
A1 Gibellini, Davide
A1 Romanelli, Maria Grazia
A1 Siccardi, Antonio
A1 Malnati, Mauro
A1 Beretta, Alberto
A1 Zipeto, Donato
A2 Medicina Física y Farmacología
A2 Grupo "Inmunología Celular y Viral"
AB HLA-C has been demonstrated to associate with HIV-1 envelope glycoprotein (Env). Virions lackingHLA-C have reduced infectivity and increased susceptibility to neutralizing antibodies. Like all othersMHC-I molecules, HLA-C requires β2-microglobulin (β2m) for appropriate folding and expression on thecell membrane but this association is weaker, thus generating HLA-C free-chains on the cell surface.In this study, we deepen the understanding of HLA-C and Env association by showing that HIV-1specifically increases the amount of HLA-C free chains, not bound to β2m, on the membrane of infectedcells. The association between Env and HLA-C takes place at the cell membrane requiring β2m tooccur. We report that the enhanced infectivity conferred to HIV-1 by HLA-C specifically involves HLA-Cfree chain molecules that have been correctly assembled with β2m. HIV-1 Env-pseudotyped virusesproduced in the absence of β2m are less infectious than those produced in the presence of β2m. Wehypothesize that the conformation and surface expression of HLA-C molecules could be a discriminantfor the association with Env. Binding stability to β2m may confer to HLA-C the ability to preferentiallyact either as a conventional immune-competent molecule or as an accessory molecule involved in HIV-1infectivity.
YR 2016
FD 2016
LK http://riull.ull.es/xmlui/handle/915/35363
UL http://riull.ull.es/xmlui/handle/915/35363
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 14-may-2024