RT info:eu-repo/semantics/article
T1 HIV-1 envelope glycoproteins isolated from Viremic Non- Progressor individuals are fully functional and cytopathic.
A1 Valenzuela Fernández, Agustín
A1 Cabrera Rodríguez, Romina
A1 Hebmann, Veronique
A1 Marfil, Silvia
A1 Pernas, María
A1 Marrero Hernández, Sara
A1 Cabrera, Cecilia
A1 Urrea, Victor
A1 Casado, Concepción
A1 Olivares, Isabel
A1 Márquez Arce, Daniel
A1 Pérez Yanes, Silvia
A1 Estévez Herrera, Judith
A1 Clotet, Bonaventura
A1 Espert, Lucile
A1 López Galíndez, Cecilio
A1 Biard-Piechaczyk, Martine
A1 Blanco, Julià
A2 Medicina Física y Farmacología
A2 Grupo "Inmunología Celular y Viral".
AB In untreated HIV-1-infected individuals, viremia is positively associated with disease progression.However, some viremic non progressors (VNPs) individuals show paradoxical high CD4+ T cell counts.HIV-1 envelope glycoprotein complex (Env) is a major cytopathic determinant in viral replication;therefore, we have deeply characterized Env function in this rare clinical phenotype. Full-length Envclones isolated from individuals with Viral Load (VL) > 10,000 copies/mL classifed as VNPs (n=15)or rapid progressors (RPs, n=17) were geno- and phenotypically analyzed by determining diversity,expression, CD4 binding/signaling, fusogenicity, infectivity and autophagy induction. Selected Envclones from VNPs and RPs (n=32) showed similar expression, fusion and infection abilities. Env clonesfrom both groups showed similar afnity for CD4 during cell-to-cell transmission and consistentlyinduced similar levels of CD4 signaling, measured by α-tubulin acetylation. Moreover, we demonstratefor the frst time that primary Env clones from VNP and RP induce autophagy in uninfected cells andthat this feature correlated with fusogenic capacity but was unrelated to disease progression. Inconclusion, our data suggest that Env clones from VNP individuals are fully functional. Therefore, theparadoxical CD4+ T cell count stability coexisting with high levels of viral replication is unrelated to Envfunction.
YR 2019
FD 2019
LK http://riull.ull.es/xmlui/handle/915/35377
UL http://riull.ull.es/xmlui/handle/915/35377
LA en
DS Repositorio institucional de la Universidad de La Laguna
RD 10-may-2024